Features: 4 photomultiplier tubes (PMTs), a low-noise HyD hybrid detector, motorized x-y stage, z-galvo, resonant scanner for high-speed imaging, and stage-top Tokai Hit environmental chamber with temperature, humidity and CO2 control.
Analysis: An off-line workstation with Leica software is available for imaging analysis.
Faculty and students who wish to use the microscope should follow the instructions in the New Users Guide. Training takes ~2 to 3 hrs, with additional mandatory follow-up sessions required until the director determines the user is capable of operating the microscope independently. Users are encouraged to bring their own samples to the training session. If none are available, prepared slides will be used. It is highly recommended to review the UMD SP5X Quickstart Guide before training begins.
The microscope is available to trained users on an equal basis. Individual users may reserve one four hour block of time during "peak" daytime hours, and an additional 6 "off-peak" hours (all other times). Investigators performing live cell imaging experiments may signup for 12 hours blocks of time after 6pm.
For a comprehensive list of policies (including BSL-2 protocols), please see the Facility SOP.
The rate schedule applies to all users and is current for the 2017/2018 academic year. Fees are used to help cover the cost of the service contract on the microscope, which is partially subsidized by the department, and to help pay for materials such as lens paper, lens cleaning solution and mercury bulbs.
Leica DMI6000 Inverted
405 diode, Argon (458, 488, 514nm), white light (470-670nm in 1nm increments)
4 PMTs, one low-noise hybrid HyD detector, 1 transmitted light PMT
10x/0.4 NA/2.2mmWD 20x/0.8 NA multi-imm/0.26mm WD 40x/1.25 NA oil/0.1mm WD 63x/1.4 NA oil/0.1mm WD 63x/1.2 NA water/0.22mm WD 63x/1.2 NA glycerol/0.28mm WD
Tokai Hit stage-top incubator with temperature, humidity, CO2 control