Inverted microscope for imaging fixed or live cells and/or bacteria.
Equipped with X-Cite lamp for fluorescence, plus standard DAPI, GFP, dsRed, CFP, and YFP filters.
Objectives: 10x, 20x LWD, 40x LWD, 100x oil.
Features: Three stage inserts (universal, 96-well plate holder, and temperature-controlled environmental chamber for 35mm dishes and chambered coverglass). 20x and 40x long-working distances lenses for viewing cells through plastic culture dishes and plates. 100x phase contrast lens for easy viewing of bacteria. Same software (Zen) as the Zeiss LSM710 confocal.
Analysis: An off-line workstation is available for imaging analysis.
Faculty and students who wish to use the microscope should follow the instructions in the New Users Guide. Training takes 30-60 minutes. Users are encouraged to bring their own samples to the training session. If none are available, prepared slides will be used.
The microscope is available to trained users on an equal basis. Individual users may reserve one four hour block of time during "peak" daytime hours, and an additional 6 "off-peak" hours (all other times).
For a comprehensive list of policies (including BSL-2 protocols), please see the Facility SOP.
The rate schedule applies to all users and is current for the 2018/2019 academic year. Fees are used to help cover the cost of the service contract on the microscope, which is partially subsidized by the department, and to help pay for materials such as lens paper, lens cleaning solution and mercury bulbs.