**The Proteomics Core is closed until a new director can be identified**
About the Facility
The Proteomics Core Facility is located in room 0109/0111 Biology-Psychology Building. The facility is equipped with a state-of-art ThermoFisher Orbitrap Fusion Lumos Tribrid mass spectrometer that is interfaced to a Dionex Ultimate3000 RSLCnano HPLC system. The facility also maintains 2 separate data stations dedicated for proteomics data processing, database searching, and generation of reports.
Services:
- Functions:
- • Several popular software packages are installed on the data station with annual maintenance contract so that the Facility and its users always have access to the latest development in the field.
- • Software packages include Proteome Discoverer, Mascot server, Seive, and Prosight PC.
- • For protein separation, the Facility maintains an Agilent 3100 OFFGEL fractionator that performs isoelectric focusing based fractionation in solution.
- • Peptide level fractionation can be achieved online with the Dionex U3000 HPLC or offline.
Services:
- The Proteomics Core Facility is a fee for service facility supported by the College of Computer, Mathematical, and Natural Sciences (CMNS).
- The facility offers service to all researchers in the region, with priority given to users from the CMNS.
- Routine service includes molecular weight determination of intact proteins by LCMS and protein identification, and protein profiling of complex samples.
- Protein samples can be submitted as lyophilized powders, solutions, or gel slices.
- Please refer to the "How to prepare your samples" page for detailed information.
Acknowledging the Proteomics Core:
- The NIH requires that all publications containing data collected on our Orbitrap Fusion Lumos, including press releases, acknowledge NIH grant support with a disclaimer such as the following: Purchasing of the orbitrap Fusion Lumos mass spectrometer was supported by Award Number 1S10OD019938 from the National Institute of Health.
- Please acknowledge the Proteomics Core in your publications and inform us of any publication that used data collected in the Proteomics Core. This will help us keep the Core around to serve you.
Contact
The Proteomics Core is currently closed until a new director is identified.
Training Opportunities
1st Annual North American Mass Spectrometry Summer School
August 6-9, 2018 Madison, Winsconsin. Deadline to apply 4/1/18
Short courses at the annual conference of the American Society for Mass Spectrometry
June 2-3, 2018, San Diego, CA
