ASI diSPIM Lightsheet

  • The ASI diSPIM (Selective Illumination Microscope) allows for dual views of fluorescently labeled samples mounted on a chambered coverglass (in liquid) and placed on an inverted microscope.
  • The method  uses two objective lenses placed at right angle in order to illuminate a thin slice of the sample perpendicular to the direction of the observation. Because only the focus plane of the sample is illuminated,  photobleaching and photoxicity are minimized, making it an ideal method for imaging living cells and small groups of cells/embryos (up to 300um sample depth) such as C. elegans
  • The diSPIM is capable of rapid volumetric imaging: up to 10 volumes per second, and (computationally-generated) isotropic resolution (<400nm with 40x 0.8NA objective).
  • A chamber with temperature and humidity control is available for live mammalian samples.
  • Excitation wavelengths include: 405, 488, 561, 640nm

New Users

Faculty and students who wish to use the microscope should follow the instructions in the New Users Guide.

Policy

The microscope is available to trained users on an equal basis. For a comprehensive list of policies (including BSL-2 protocols), please see the Facility SOP.

System Specifications

Microscope Stand
Inverted MIM (modular infinity microscope)
Illumination
405, 488, 561, 640nm lasers
Emission
Long-pass emission filters
Light detector
Two ZYLA 4.2 sCMOS cameras
Objective Lenses
10x, 20x, 40x (all water immersion dipping lenses)
Incubation
Chamber available for temperature and CO2 control
X-Y Stage
Motorized
Sample chamber
Fits only the following size cover glass: 24 x 50mm, #1.5
Software
Micromanager

References

Contact Us

Amy Beaven
Interim Director
abeaven@umd.edu
301.405.7238
0107 Microbiology Building
Dr. Charles Delwiche
Imaging Facilities Supervisor
delwiche@umd.edu
301.405.8286
2108 Bioscience Research Building