Features: 5 light detectors: 3 HyD S and 2 HyD X (for FLIM), motorized x-y stage, z-galvo, resonant scanner for high-speed imaging, and stage-top Tokai Hit environmental chamber with temperature, humidity and CO2 control
Note: the NIH requires that all publications containing data collected on the Zeiss LSM 980 Airyscan, including press releases, acknowledge NIH grant support with a disclaimer such as the following: Purchase of the Leica Stellaris 8 FALCON was supported by Award Number 1S10ODO34260 from the National Institute of Health.
Faculty and students who wish to use the microscope should follow the instructions in the New Users Guide. Training takes ~2 to 3 hrs, with additional mandatory follow-up sessions required until the director determines the user is capable of operating the microscope independently. Users are encouraged to bring their own samples to the training session. If none are available, prepared slides will be used.
The microscope is available to trained users on an equal basis. Individual users may reserve one four hour block of time during "peak" daytime hours, and an additional 6 "off-peak" hours (all other times). Investigators performing live cell imaging experiments may signup for 12 hours blocks of time after 6pm.
For a comprehensive list of policies (including BSL-2 protocols), please see the Facility SOP.
The rate schedule applies to all users and is current for the 2022/2023 academic year. Fees are used to help cover the cost of the service contract on the microscope, which is partially subsidized by the department, and to help pay for materials such as lens paper, lens cleaning solution and mercury bulbs.
Leica inverted DMI8
405 diode and white light (440-790nm in 1nm increments)
3 HyD S, 2 HyD X, 1 transmitted light PMT
10x/0.4 NA 20x/0.75 NA 40x/1.3 NA oil 63x/1.4 NA oil 63x/1.2 NA water/0.22mm WD
Tokai Hit stage-top incubator with temperature, humidity, CO2 control