Leica Stellaris 8 FALCON Laser Scanning Confocal - New 6/30/23

  • Ideal for imaging diverse fluorophores in fixed and live samples.
  • Suitable applications include colocalization studies, fluorescence lifetime imaging (FLIM), FLIM/FRET, tile-scanning, spectral imaging, and time courses.
  • Lasers: 405nm and white light (440-790nm in 1nm increments).
  • Objectives:  10x, 20x, 40x oil, 63x oil, 63x water.
  • Additional features:
    • 5 light detectors: 3 HyD S and 2 HyD X (for FLIM)
    • Motorized x-y stage
    • Z-galvo stage
    • Conventional and resonant scanner for high-speed imaging
    • Stage-top Tokai Hit environmental chamber with temperature, humidity and CO2 control

New Users

Note: the NIH requires that all publications containing data collected on the Leica Stellaris 8, including press releases, acknowledge NIH grant support with a disclaimer such as the following: Purchase of the Leica Stellaris 8 FALCON was supported by Award Number 1S10ODO34260 from the National Institute of Health. 

Faculty and students who wish to use the microscope should follow the instructions in the New Users Guide. Training takes ~2 to 3 hrs, with additional mandatory follow-up sessions required until the director determines the user is capable of operating the microscope independently. Users are encouraged to bring their own samples to the training session. If none are available, prepared slides will be used.

Policy

The microscope is available to trained users on an equal basis. Individual users may reserve one four hour block of time during "peak" daytime hours, and an additional 6 "off-peak" hours (all other times). Investigators performing live cell imaging experiments may signup for 12 hours blocks of time after 6pm.

For a comprehensive list of policies (including BSL-2 protocols), please see the Facility SOP.

Rates

The rate schedule applies to all users and is current for the 2023/2024 academic year. Fees are used to help cover the cost of the service contract on the microscope, which is partially subsidized by the department, and to help pay for materials such as lens paper, lens cleaning solution and mercury bulbs.

System Specifications

Microscope Stand
Leica inverted DMI8
Illumination
405 diode and white light (440-790nm in 1nm increments)
Emission
Fully spectral
Light detector
3 HyD S, 2 HyD X, 1 transmitted light PMT
Objective Lenses
10x/0.4 NA
20x/0.75 NA
40x/1.3 NA oil
63x/1.4 NA oil
63x/1.2 NA water/0.22mm WD
Incubation
Tokai Hit stage-top incubator with temperature, humidity, CO2 control
X-Y Stage
Motorized with z-galvo
Software
Leica LAS X

References

References:

Contact Us

Amy Beaven
Facility Director
[email protected]
301.405.7238
0107 Microbiology Building
Dr. Charles Delwiche
Faculty Supervisor
[email protected]
301.405.8286
2108 Bioscience Research Building