Zeiss Lattice Lightsheet  7

  • The Zeiss Lattice Lighsheet 7 (LLS7)  is optimized for rapid live-cell imaging in four dimensions (x, y, z and time).
  • The system is capable of high-speed volumetric imaging, and produces high signal-to-noise ratio images with minimal photobleaching.
  • It is equipped with 2 Hamamatsu ORCA-Fusion cameras for 2-color simultaneous imaging, three solid-state lasers ( 488, 561, 640nm), an Ibidi environmental chamber with temperature, humidity, and C02 control, and a motorized x-y-z/tilt stage. 
  • Suitable for adherent cells, spheroids, organoids, and small evolving organisms.

New Users

Faculty and students who wish to use the microscope should follow the instructions in the New Users Guide. Training takes ~2 to 3 hrs, with additional mandatory follow-up sessions. Users are encouraged to bring their own samples to the training session. If none are available, prepared slides will be used.

Policy

The microscope is available to trained users on an equal basis. An individual user may reserve one four hour block of time during "peak" daytime hours, and an additional 6 "off-peak" hours (all other times). Investigators performing live cell imaging experiments may signup for overnight blocks of time after 6pm.

For a comprehensive list of policies (including BSL-2 protocols), please see the Facility SOP.

Rates

The rate schedule applies to different users and is current for the 2022/2023 academic year. Fees are used to help cover the cost of the service contract on the microscope, and to help pay for materials such as lens paper, lens cleaning solution and mercury bulbs.

System Specifications

Microscope Stand
Zeiss Inverted
Illumination
Lasers: 488, 561, 640nm
LED: for finding sample
Emission
Camera 1
• BP 570-620 + LP 655
• BP 495-550 + LP 655
• LBF 405/488/561/642
• ND filter
• BP 495-570
• LP 570

Camera 2
• BP 570-610 IR+
• BP 495-550 + BP 570-620
• BP 500-550 IR+
Light detectors
2 Hamamatsu ORCA-Fusion cameras for simultaneous 2-color imaging
Objective Lenses
Illumination: 13.3x/NA 0.4
Detection: 44.83x/NA 1.0
Incubation
Ibidi stage-top incubator with temperature, humidity, CO2 control. Can accommodate 35mm dishes, chambered coverglass, and 3, 6, 12-well plates with coverglass bottoms.
X-Y Stage
Motorized
Software
Zen 3.6 (blue edition)
Lattice Lightsheet Processing Module (and analysis computer)

References

Imaging Incubator Protocols:Useful Links:

Contact Us

Amy Beaven
CBMG Imaging Core Director
abeaven@umd.edu
301.405.7238
0107 Microbiology Building