The DeltaVision Elite Deconvolution/TIRF microscope system is a 3-D deconvolution (image restoration) system with 488nm and 561nm laser lines for TIRF (total internal reflection microscopy).
Deconvolution uses the properties of the microscope system (point spread function) to deblur and remap out-of-focus fluorescent signal in order to enhance image resolution and contrast. TIRF is a technique used to image samples within ~100-200nm of the coverslip surface.
The system is equipped a number of objective lenses (including 60x oil, 60x water and 60x TIRF), a CMOS camera, and DAPI, CFP, GFP, YFP, TRITC, mCherry, and CY5 fluorescence filters. It is recommended for fluorescence imaging of thin fixed specimens and time-lapse imaging of live cells. The proprietary solid state illumination system provides uniform, high intensity light to the sample, enabling detection of small, dim objects, and allowing for shorter exposure times and fast image acquisition. An incubation system with temperature control and 5% CO2 is available for long-term live cell imaging experiments. The system is equipped with an Ultimate Focus laser that can help prevent z-drift during time-lapse studies.
When in TIRF mode (excitation 488, 561nm), the system images samples within 100-200nm of the coverslip surface. The TIRF angle is variable and each wavelength is chromatically corrected to ensure both laser lines penetrate the sample to the same depth.
0107 Microbiology Building
Dr. Charles Delwiche
2108 Bioscience Research Building