NEW USER GUIDE

Instructions for Prospective Imaging Core Users

If you aren't sure which microscope to use (Leica SP5X confocal, Zeiss LSM710 confocal, DeltaVision Elite/TIRF, Zeiss AxioObserver, Nikon Eclipse), contact Amy Beaven.
Decide if you'd like to a) be fully trained to operate the microscope independently, or b) have the director operate the microscope for you. For information on microscope hourly rates and training fees, please see the Rate Schedule.
All prospective users must apply online (below), or download an Application Form, fill it out in detail, and email it to Amy Beaven (abeaven@umd.edu). If you plan to image live BSL-2 samples, make sure to review the Facility SOP and provide the necessary information indicated in the application form. Upon receipt of the completed application, you will be contacted within 2 days.
Typical training times: 2-3 hrs for confocal and DeltaVision; 30-60 min for the AxioObserver and Eclipse. Users are highly encouraged to bring their own samples to the training session. Follow-up sessions are required for the confocals and Deltavision. Users may not operate these microscopes independently until they receive permission from the Director. For a complete list of policies and procedures, see the Facility SOP.
Scheduling is accomplished using Google Calendar. You will receive an invite to an instrument's Google calendar once a valid gmail account is provided.
Prospective microscope users should review Facility SOP, as well as one of the following guides: the Leica SP5X Quickstart Guide, Zeiss LSM710 Quickstart Guide or DeltaVision Quickstart Guide before attending the first training session.
Arrive on time for your training session (0107 Microbiology building). If you are more than 15 minutes late, the training session will be canceled and you will be charged at the regular rate for the full length of the appointment. Cancellations made less than 24 hours in advance will also be charged at the regular rate.
Note: It is important to properly acknowledge the use of the Imaging Core in your publications. Acknowledgement helps us demonstrate and document how the Imaging Core contributes to the research community. It also aids us in our efforts to secure more funding to purchase additional instruments and offer new services. Suggested wording: "We acknowledge the Imaging Core Facility in the department of Cell Biology and Molecular Genetics at the University of Maryland--College Park for [name of instrument; brief description of assistance]. Imaging Core users should send a PDF copy of all publications to the director, Amy Beaven.

Useful Links:

Application for Use of Imaging Core Microscopes

* Indicates required field

Name *

First

Last

UID# (Non-UMD external users: enter the name of your institution/company) *

Email Address *

Status (select one) *

Department Affiliation *

Room # and Building *

Phone Number *

Lab Head *

Google Calendar Address for Instrument Scheduling *

KFS/FRS# for internal billing. (External users, write "credit card") *

Application Type (select one) *

1. Briefly describe your experience with any of the following microscopy techniques: fluorescence, confocal, deconvolution, TIRF, lightsheet, AFM (or other) *

2. Which microscope do you need to use (if you require training on additional microscopes, please let the director know at the time of your appointment) *

3. Briefly describe your research project and why you require use of the microscope *

4. How soon do you require training or assisted-microscope-use? Note: Users are highly encourage to have a sample ready for training. *

5. At any time will you be working with LIVE biological agents/organisms (including bacterial, viral, fungal, human/non-human primates) or toxins? *

Yes No

6. If yes, please describe the agent: *

7. Is the LIVE agent BSL-1 or BSL-2? *

BSL-1 BSL-2 N/A: My sample is fixed (dead)

Please note: If you'd like to image live BSL-2 samples, you must submit 1) an approved protocol for your agent (including safe cleanup procedures) and 2) an IBC approval letter prior to your appointment. You may use the form below to upload the files, or email them separately to Amy Beaven (abeaven@umd.edu).

*Human and non-human primate cell lines are considered BSL-2. Any work involving recombinant or synthetic genomic material, infectious agents, or animals with rDNA will require registration and approval of the Institutional Biosafety Committee (IBC). Even if you have an approved IBC protocol, you may need to amend and resubmit it for use any Imaging Core, particularly if you are bringing samples from another building.*

8. Upload BSL-2 protocol *

Max file size: 20MB

9. Upload IBC approval letter *

Max file size: 20MB

Submit

Contact Us

Amy Beaven
Facility Director
abeaven@umd.edu
301.405.7238
0107 Microbiology Building

Dr. Charles Delwiche
Faculty Supervisor
delwiche@umd.edu
301.405.8286
2108 Bioscience Research Building