Instrument
Style
Type
Objectives
Sample Type
Stage
Incubation
Relative Speed
Lasers/ Filters
Zeiss LSM710
Inverted
Laser scanning confocal fluorescence
5, 10, 40x oil, 63x oil,
100x oil
Live or fixed cells or tissues
Manual
None
Slower
405, 458, 488, 514, 561, 633nm
Leica SP5X
Inverted
Laser scanning confocal fluorescence
10, 20, 40x oil, 63x oil, 63x water, 63x glyc
Live or fixed cells or tissues
Motorized in x,y,z
Tokai Hit Stagetop with CO2
Slower
405, argon (458, 488, 514), white light laser (470-670nm)
DeltaVision Deconvolution/TIRF
Inverted

Deconvolution/
Total Internal Reflection fluorescence
10x, 20x LWD, 40x LWD, 60x oil, 60x water, 60x TIRF
Live or fixed cells
Motorized in x,y,z
"Big box" Incubator with CO2
Faster
DAPI/GFP/DsRed/A633
or
CFP/YFP/mCherry

TIRF: 488, 561nm lasers
Zeiss AxioObserver
Inverted
Widefield fluorescence
10x, 20x LWD, 40x LWD, 100x oil
Live or fixed cells, suitable for cells in plastic plates
Manual
Stagetop Incubator, no CO2
Faster
Standard DAPI, GFP, dsRed, CFP, YFP filters
Nikon Eclipse
Upright
Brightfield with color camera,
fluorescence with monochrome camera
4x, 10x, 20x, 40x oil
Live or fixed cells or tissue
Motorized x-y
None
Faster
DAPI, GFP, dsRed (manual switching)

For all your light microscopy needs....

The Imaging Core (IC) provides researchers with the opportunity to image live, fixed, unlabeled or fluorescently labeled samples using one of several available microscopes. Services include training on all equipment, guidance on experimental design, and technician-assisted microscope operation.

Leica SP5X Laser Scanning Confocal

Ideal for imaging diverse fluorophores in fixed and live samples. Lasers include: 405nm, argon (458, 488, 514nm), and White Light (470-670nm, tunable in 1nm increments). System is equipped with a low-noise hybrid detector, motorized x-y stage, environmental chamber, and resonance scanner for high-speed imaging.

Zeiss LSM710 Laser Scanning Confocal

Ideal for imaging fixed, live (room temperature), single or multi-labeled samples. System is equipped with a manual stage, 3 light detectors, and 6 major laser lines (405, 458, 488, 514, 561, 633nm) Suitable applications include colocalization studies, immunofluorescence, FRAP and time courses.

DeltaVision Elite Deconvolution/TIRF

The DeltaVision is a 3-D deconvolution system with 488 and 561nm lasers for TIRF (total internal reflection microscopy). Features include motorized x-y stage, environmental chamber, and epi filters for DAPI, CFP, GFP, YFP, dsRed, mCherry, Cy5. The system excels at imaging "small, dim, live" samples.

Zeiss AxioObserver Fluorescence

Inverted microscope equipped phase contrast, standard fluorescence filters (DAPI, GFP, DsRed, CFP, YFP), and monochrome camera. Suitable for imaging of bacteria and thin fluorescently labeled samples. Two long-working distances lenses (20, 40x) and 96-well plate stage adaptor allow for imaging of cells in culture.

    Application for Use of Imaging Core Microscopes
     Please note: If you'd like to image live BSL-2 samples, you must email 1) an approved protocol for your agent (including safe cleanup procedures) and 2) IBC approval letter to Amy Beaven ([email protected]) prior to your training appointment. *Human and non-human primate cell lines are considered BSL-2. Any work involving recombinant or synthetic genomic material, infectious agents, or animals with rDNA will require registration and approval of the Institutional Biosafety Committee (IBC). Even if you have an approved IBC protocol,  you may need to amend and resubmit it for use any Imaging Core, particularly if you are bringing samples from another building.*

Contacts

email
[email protected]
[email protected]
​

phone
415.555.2530
415.555.9932​

address
1523 Market St  Suite 100
​San Francisco, CA 94118